PENGARUH LENDIR BEKICOT (Achatina fulica) TERHADAP JUMLAH FIBROBLAS PADA SOKET
DOI:
https://doi.org/10.21776/ub.eprodenta.2020.004.02.1Keywords:
Lendir Bekicot (Achatina fulica), sel fibroblast, penyembuhanAbstract
Latar belakang : Penyembuhan luka adalah proses dinamis yang menghasilkan serangkaian aktivitas termasuk peradangan, proliferasi, dan migrasi sel-sel fibroblast ke daerah luka. Fibroblas sangat penting dalam mendukung penyembuhan luka normal, dari fase akhir inflamasi hingga fase akhir epitelisasi pada jaringan yang mengalami jejas. Tujuan : Penelitian ini mengamati pengaruh lendir bekicot (Achatina fulica) dalam mempercepat proses penyembuhan melalui proliferasi fibroblas di soket paska pencabutan gigi. Metode : Tikus wistar (Rattus novergicus) dibagi menjadi dua kelompok, dan gigi insisivus pertama rahang bawah kiri mereka dicabut. Soket bekas pencabutan diberikan lendir bekicot pada kelompok perlakuan dan kelompok kontrol tidak diberikan lendir bekicot. Pada hari ke 3, 5 dan 7, tikus dilakukan eutanasia dan sampel jaringan diolah untuk analisis histologi. Hasil : Uji statistik penelitian menunjukkan bahwa lendir bekicot (Achatina fulica) secara signifikan meningkatkan jumlah sel fibroblas pada kelompok perlakuan dibandingkan dengan kelompok kontrol (p<0,05). Kesimpulan : Dari penelitian ini dapat disimpulkan bahwa lendir siput (Achatina fulica) mempunyai potensi yang cukup baik dalam merangsang proses penyembuhan luka setelah pencabutan gigi.References
Addis R, Cruciani S, Santaniello S, et al. Fibroblast proliferation and migration in wound healing by phytochemicals: Evidence for a novel synergic outcome. Int J Med Sci. 2020;17(8):1030-1042. doi:10.7150/ijms.43986
Guo S, DiPietro LA. Critical review in oral biology & medicine: Factors affecting wound healing. J Dent Res. 2010;89(3):219-229. doi:10.1177/0022034509359125
Desjardins-Park HE, Foster DS, Longaker MT. Fibroblasts and wound healing: an update " Increased comprehension of dermal fibroblast heterogeneity may yield both mechanistic insights into existing therapies and inspiration for novel therapeutics targeting specific cell populations in wound heal. Regen Med. 2018;13:491-495. doi:10.2217/rme-2018-
Gonzalez AC de O, Costa TF, Andrade Z de A, Medrado ARAP. Wound healing - A literature review. An Bras Dermatol. 2016;91(5):614-620. doi:10.1590/abd1806-4841.20164741
Adi P, Aulia Hapsari Y, Nurin Nafilah A, Arifin Z. Jumlah Fibroblas Dan
Angiogenesis Setelah Pemberian Gel Getah Jarak Cina Pada Ulserasi Tikus Wistar. EProdenta J Dent. 2019;3(1):180-186. doi:10.21776/ub.eprodenta.2019.003.01.1
Park Y. Mining invertebrate natural products for future therapeutic treasure. Nat Prod Commun. 2011;6(9):1403-1408.
Jayashankar M, Ghosh SK, Devaiah MK, Pradeep AS, Reddy MS, Ramakarishna S. Studies to assess the mucus of giant African snail Achatina fulica ( Bowdich ) as an ovipositional attractant for mosquitoes.
J Entomol Zool Stud. 2014;2(6):82-85.
Berniyanti T, Suwarno. Karakterisasi Protein Lendir Bekicot (Achasin) Isolat Lokal sebagai Faktor Antibakteri. Media Kedokt Hewan. 2007;23(3):139-144.
Vieira TCRG, Costa-Filho A, Salgado NC, et al. Acharan sulfate, the new glycosaminoglycan from Achatina fulica Bowdich 1822: Structural heterogeneity, metabolic labeling and localization in the body, mucus and the organic shell matrix. Eur J Biochem. 2004;271(4):845-854.
doi:10.1111/j.1432-1033.2004.03989.x
Harti AS, Murharyati A, Dwi Sulisetyawati S, Oktariani M. The effectiveness of snail mucus (Achatina fulica) and chitosan toward limfosit proliferation in vitro. Asian J Pharm Clin Res. 2018;11(Special Issue 313 3):85-88. doi:10.22159/ajpcr.2018.v11s3.30041
Anderson K, Hamm RL. Factors that impair wound healing. J Am Coll Clin Wound Spec. 2014;4(4):84-91. doi:10.1016/j.jccw.2014.03.001
Rahman MNA, Sukmasari S, Doolaanea AA, Qader OAJA. Potential oral wound healing of topical application of dental gel prepared from Baccaurea angulata fruit in diabetic rats. J Pharm Sci Res. 2018;10(1):167-174.
Machmud E, Ruslin M, Waris R, Asse RA, Qadafi AM, Achmad H. Effect of the application of chlorella vulgaris ointment to the number of fibroblast cells as an indicator of wound healing in the soft tissue of pig ears. Pesqui Bras Odontopediatria Clin Integr. 2020;20:1-10. doi:10.1590/pboci.2020.032
Sodipe OG, Osinowo OA, Onagbesan OM, Bankole MO. EVALUATION OF THE HAEMOLYMPH OF THE GI- ANT AFRICAN
LAND SNAILS Achatina achatina AND Archachatina marginata FOR BACTERIA STERILITY AND INHIBITORY PROPERTIES. J Agric Sci Environ. 2013;13(June 2016):10-14.
Jeong J, Toida T, Muneta Y, et al. Localization and characterization of
acharan sulfate in the body of the giant African snail Achatina fulica. 2001:513-519.
Vieira AE, Repeke CE, De Barros Ferreira S, et al. Intramembranous bone healing process subsequent to tooth extraction in mice: Micro-computed tomography, histomorphometric and molecular characterization. PLoS One. 2015;10(5):1- 22. doi:10.1371/journal.pone.0128021
Young E, Godwin Determination Of Nutrients / Organic Compounds In The Foot And Slime Of Land Snail ( Achatina
Achantina ). Am J Eng Res. 2019;8(7):27- 31.
Wong T, McGrath JA, Navsaria H. The role of fibroblasts in tissue engineering and regeneration. Br J Dermatol. 2007;156(6):1149-1155.
doi:10.1111/j.1365-2133.2007.07914.x
Ten Dam GB, Van De Westerlo EMA, Smetsers TFCM, et al. Detection of 2-Osuifated iduronate and Nacetylglucosamine units in heparan sulfate by an antibody selected against acharan sulfate (IdoA2S-GlcNAc)n. J Biol Chem. 2004;279(37):38346-38352.
doi:10.1074/jbc.M404166200
Wang H, Toida T, Kim YS, et al. Glycosaminoglycans Can Influence
Fibroblast Growth Factor-2 Mitogenicity without Significant Growth Factor Binding. Biochem Biophys Res Commun. 1997;235(2):369-373.
doi:doi.org/10.1006/bbrc.1997.6789
Lin X. Functions of heparan sulfate proteoglycans in cell signaling during development. Development. 2004;131(24):6009-6021.
doi:10.1242/dev.01522
Simon Davis DA, Parish CR. Heparan sulfate: A ubiquitous glycosaminoglycan with multiple roles in immunity. Front Immunol. 2013;4(DEC):1-7. doi:10.3389/fimmu.2013.00470
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