PENGARUH LAMA PAPARAN LPS (LIPOPOLISAKARIDA) Phorpyromonas gingivalis SEBAGAI INDUKTOR PERIODONTITIS TERHADAP KADAR LDL DAN HDL TIKUS WISTAR (Rattus norvegicus)
DOI:
https://doi.org/10.21776/ub.eprodenta.2017.001.02.2Abstract
Penyakit kardiovaskular termasuk penyakit paling mematikan di dunia dan salah satu yang utama adalah aterosklerosis. Peningkatan kadar low density lipoprotein (LDL) dan penurunan kadar high density lipoprotein (HDL) dalam darah merupakan faktor risiko terjadinya aterosklerosis. Paradigma baru menjelaskan bahwa inflamasi kronis seperti periodontitis dipertimbangkan menjadi faktor risiko terjadinya aterosklerosis. Bakteri penyebab periodontitis terutama adalah Porphyromonas gingivalis. Tujuan penelitian ini untuk mengetahui pengaruh lama paparan LPS Phorpyromonas gingivalis sebagai induktor periodontitis terhadap peningkatan kadar LDL dan penurunan kadar HDL tikus wistar. Penelitian ini menggunakan True Experimental Design yaitu Posttest Control Group Design. Lama paparan LPS Phorpyromonas gingivalis yang digunakan yaitu 28 hari dan 60 hari. Hasil penelitian menunjukkan tidak ada perbedaan yang signifikan antara lama paparan LPS Phorpyromonas gingivalis terhadap peningkatan kadar LDL dan penurunan kadar HDL tikus wistar baik pada 28 hari maupun 60 hari terhadap kelompok kontrol. Analisa data dengan menggunakan Uji One-Way ANOVA menunjukkan nilai signifikansi ɑ = 0.441 pada LDL dan ɑ = 0.982 pada HDL (ɑ ˃0.05). Uji korelasi Pearson menunjukkan bahwa semakin lama paparan LPS Phorpyromonas gingivalis sebagai induktor periodontitis akan cenderung meningkatkan kadar LDL dan menurunkan kadar HDL tikus wistar. Berdasarkan penelitian ini, dapat disimpulkan bahwa lama paparan LPS Phorpyromonas gingivalis selama 28 hari dan 60 hari sebagai induktor periodontitis cenderung akan meningkatkan kadar LDL dan menurunkan kadar HDL tikus wistar.
Â
Kata kunci: periodontitis, lipopolisakarida, Porphyromonas gingivalis, kadar LDL, kadar HDL
References
Beck JD, Offenbacher S. 2001. The association between periodontal diseases and cardiovascular diseases: a state-of-the science review. Ann Periodontol 2001; 6: 9-15.
Nugraha MA, Suci D AW, Susilawati IDA. 2014. Kadar LDL dan HDL Dalam Darah Model Tikus Periodontitis (Blood Level of LDL and HDL in Periodontitis Rat Model ). e-Jurnal Pustaka Kesehatan, vol. 2 (no.1), Januari.
Permana R, Rizqi F, Pradana A, Susilawati IDA, Ermawati T. 2013. Analisis Histomorfometrik Pembentukan Lesi Aterosklerosis Koroner pada Model Tikus (Rattus norvegicus) Periodontitis (Histomorphometric Analysis of Coronary Atherosclerosis Lesions in Periodontitis Rat (Rattus norvegicus) Model). Journal of Dentistry Indonesia, Vol. 20, No. 3, 73-77.
Rose LF, Mealey BL. 2004. Periodontics: medicine, surgery, and implants. Saint Louis: Elsevier Mosby.
US Census Bureau. Periodontitis in Asia (Extrapolated Statistics). 2004. International database: 2004 [cited 2015 January 12. Available from: http://www.CureResearchcom.htm.
Roeslan BO, Hidayat MA, Rahmadi H, Prasetyo B, Ermayanti. 2003. Kadar IgA dan IgG dalam saliva penderita penyakit periodontal sebelum dan sesudah operasi. Jurnal PDGI. 2003;53(2):25-9.
Haake SK, Newman MG, Nisengard RJ, Sanz M. 2002. Periodontal microbiology. In: Newman MG, Takei HH, Carranza FA, editors. Carranza’s clinical periodontology 9th ed. Sydney; p.106.
Manson J.D., Eley B.M. 2004 Periodontics, Fifth Edition, Edinburgh London New York etc: Wright.
Carranza FA, Takei HH. 2006. Carranza’s Clinical Perioidontology 10th ed. Missouri: Saunders Elsevier.
Kido J, Kido R, Suryono, Kataoka M, Fagerhol MK, Nagata T. 2004. Induction of calprotectin released by porphyromonas gingivalis lipopolysaccharide in human neutrophil. Oral Microbiology and Immunology. 19:182-7.
Harazy VI., Zambon JJ., Trevisan M., Zeid M., Genco RJ. 2000. Identification of Periodontal Pathogens in Atheromathous Plaques. J. Periodontal. 71:1554-60.
Paquette DW, Brodala N, Nichols TC. 2007. Cardiovascular disease, inflammation and periodontal infection. Periodontology 2000. Vol. 44 : 113-26.
Susanto, A., Rusyanti, Y. 2010. Penyakit periodontal dan penyakit jantung koroner (aterosklerosis) Available from:pustaka.unpad.ac.id/wp-content/uploads/.../penyakit_periodontal.pdf, diakses Januari 2015.
R.M. Sandi, K.G. Pol, P. Basavaraj, Nitin Khuller, Shilpi Singh. 2014. Association of Serum Cholesterol, Triglyceride, High and Low Density Lipoprotein (HDL and LDL) Levels in Chronic Periodontitis Subjects with Risk for Cardiovascular Disease (CVD): A Cross Sectional Study. Journal of Clinical and Diagnostic Research. 2014 Jan, Vol-8(1): 214-216.
Rashidinejad H, Mehrizi H, Arsalan A, Rahmani MR and Ahmadi J. 2009. Effect of Periodontal Diseases on Plasma Level of LDL, HDL and Total Cholesterol in Rats. Iranian Heart Journal.10 (1):48-5.
Sudrajat, J. 2008. Profil Lemak, Kolesterol Darah, dan Respon Fisiologis Tikus Wistar yang Diberi Ransum Mengandung Gulai Daging Sapi Lean. Skripsi. Tidak diterbitkan, Fakultas Peternakan Institut Pertanian Bogor, Bogor.
Megasari, 2009,. Pengaruh Lama Stres dan diet Aterogenik terhadap Pembentukan Foam Cell pada Arteri Koroner Jantung Tikus Putih (Rattus novergicus galur Sprague Dawley) Jantan. Universitas Islam Negeri (UIN) Maulana Malik Ibrahim, Malang.
Marks DB., Marks AD., Smith CM. 2000. Basic medical biochemistry : a clinical approach. Biokimia Kedokteran Dasar : Sebuah pendekatan klinis, 2000, Brahm U. Pendit (alih bahasa), Jakarta : EGC.
Hendra AR, Riskyana Dwi. 2015. Profil Lipid Darah pada Model Tikus Pulpitis dengan Paparan Streptococcus mutans. Skripsi. Tidak diterbitkan, Fakultas Kedokteran Gigi Universitas Jember, Jember.
Naqvi AR, Fordham JB, Khan A, Nares S. 2015. microRNAs responsive to A.actinomycemcomitans and P.gingivalis LPS modulate expression of genes regulating innate immunity in human macrophages. HHS Public Access. Innate Immun. Author manuscript : 540-551.
Ebrahem MA. 2013. Expression of human beta defensins (HBDs) 1, 2, and 3 in gingival crevicular fluid of patients affected by localized aggressive periodontitis. The Saudi Dental Journal (2013) 25, 75-82.
Miyazawa H, Tabeta K, Miyauchi S, Aoki-Nonaka Y, Domon H, Honda T, Nakajima T dan Yamazaki K. 2012. Effect of Porphyromonas gingivalis infection on post-transcriptional regulation of the low-density lipoprotein receptor in mice. Miyazawa et al. Lipids in Health and Disease. 11:121
Thomas E. Van Dyke, Kenneth S. Kornman. 2008. Inflammation and FactorsThat May Regulate Inflammatory Response. J Periodontol August 2008 Volume 79 Number 8; 1503-1507.
Palacio C, Alexandraki I, Bertholf RL, Mooradian AD. 2011. Transient Dyslipidemia Mimicking the Plasma Lipid Profile of Tangier Disease in a Diabetic Patient with Gram Negative Sepsis. Ann Clin Lab Sci Spring 2011 vol 41 no. 2 150-153.
Murray JA., Wilton JMA. 2003. LPS from Periodontal Pathogen P. gingivalis Prevents Apoptosis of HL50-Derived Neutrophils in Vitro. J Infect Immun: 71(12): 7232-7235.
Djohari M, Syamsu. 2009. Modified Low Density Lipoprotein (LDL) In Atherogenesis Process. The Indonesian Journal Of Medical Science Volume 1 No.8 April 2009 P. 502-510
Shibata N, Christopher K Glass. 2009. Regulation of macrophage function in inflammation and atherosclerosis. Journal Lipid Research. April 2009. 50(Suppl): S277–S281
Downloads
Published
How to Cite
Issue
Section
License
E-Prodenta Journal of Dentistry receives original texts that have not been published in any media in any language, or are being sent to other journals at the same time. If the manuscript has already been presented in a particular symposium or seminar, then it should be written in the description. The submission of the manuscript shall be accompanied by a statement of authenticity of writing and unpublished statements made and signed by the first author. When the manuscript is accepted for publication, the authors agree to automatic transfer of the copyright to the publisher.
