EFEK EKSTRAK DAUN TEH HIJAU (Camellia sinensis) TERHADAP SEL OSTEOKLAS TULANG ALVEOLAR TIKUS PUTIH (Rattus norvegicus)
DOI:
https://doi.org/10.21776/ub.eprodenta.2018.002.01.5Keywords:
Ekstrak daun teh hijau, Osteoklas, Lipopolisakarida, tulang alveolarAbstract
ABSTRAK
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Periodontitis merupakan keradangan pada jaringan pendukung gigi yang menyebabkan kerusakan tulang alveolar. Bakteri Aggregatibacter actinomycetecomitans menghasilkan  Lipopolisakarida (LPS) yang dapat meningkatkan aktivitas osteoklas. Teh hijau memiliki kandungan epigallocatechin gallate (EGCG) yang dapat menurunkan aktivitas dan diferensiasi sel osteoklas dengan menghambat peningkatan RANKL. Tujuan: untuk mengetahui efek  ekstrak daun teh hijau (Camellia sinensis) terhadap penurunan jumlah sel osteoklas tulang alveolar tikus putih (Rattus norvegicus) dengan diinduksi lipopolisakarida. Metode: Tikus putih sebanyak 28 ekor dibagi dalam 4 kelompok, yaitu Kelompok K- (tidak diberi perlakuan), Kelompok K+ (diinduksi LPS Aa), Kelompok P1 dan Kelompok P2 (masing-masing diinduksi LPS Aa dan diberi ekstrak daun teh hijau dengan dosis 150 mg / 100 gram BB dan 200 mg / 100 gram BB). Tikus didekaputasi untuk diambil tulang alveolar rahang bawah dan dilakukan pengamatan sel osteoklas dengan pewarnaan HE. Hasil: terdapat perbedaan jumlah sel osteoklas yang signifikan antar kelompok (One Way Anova, p<0,05) dan tidak terdapat perbedaan jumlah sel osteoklas yang signifikan antar kelompok P1 dan P2 (Post Hoc, p>0,05). Kesimpulan: ekstrak daun teh hijau (Camellia sinensis) mempunyai efek terhadap penurunan jumlah sel osteoklas tulang alveolar tikus putih (Rattus norvegicus) dengan diinduksi lipopolisakarida dimana dosis efektif ekstrak daun teh hijau 150mg/100grBB.
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Kata Kunci : Ekstrak daun teh hijau, Osteoklas, Lipopolisakarida, tulang alveolar
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ABSTRACT
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Periodontitis is an inflammation in teeth supporting tissue thus allowing the destruction in alveolar bone. Aggregatibacter actinomycetecomitans bacteria which produces lipopolysaccharide (LPS) to increase osteoclast production. Green tea contains epigallocatechin gallate (EGCG) which is able to decrease the activity and differentiation of osteoclast by obstructing the increase of RANKL expression. Objectives: To determine the effects of green tea (Camelia Sinensis) extract towards decreasing number of osteoclast in white rat’s (Rattus Norvegicus) socket which is induced by lipopolysaccharide. Methode: the 28 rats is divided into 4 groups. The groups are K- group (the experimental animals weren’t given any treatment), K+ group (the experimental animals were induced by Aa’s LPS), P1 and P2 group (the experimental animals were induced by Aa’s LPS and with 150 mg/100 g and 200 mg/100 g weight of green tea extract).The rats will be sacrificed and dissected to take its lower jaw’s alveolar bone. Then HE staining is done to observe the osteoclast. Result: There is a significant difference in osteoclast number in each group. (The One-way ANOVA, (p<0,05). There is no significant difference in osteoclast cell count between P1 and P2 groups (Post Hoc, p>0,05). Conclusion: The green tea (Camellia Sinensis) extract has an effect towards the decrease of osteoclast number on White Rat’s (Rattus norvegicus) alveolar bone which is induced with lipopolysaccharide, and the effective dose is 150 mg/100 g weight. Keywords: Green Tea Lesf Extract, Osteoclast, Lipopolysaccharide, Alveolar BoneReferences
Dharmayanti, Agustin. 2012. Deoxypyridinoline Level in Gingival Crevicular Fluid as Alveolar Bone Loss Biomarker in Periodontal Disease. J KG Unej; 2(3): 2-6.
Dinas Kesehatan Kota Malang. 2009. Laporan Bulanan Kegiatan Puskesmas Tahun 2009. Tidak Diterbitkan.
Laine ML, Crielaard W, Loos BG. 2012. Genetic Susceptibility to Periodontitis. Periodontal J. 58(1):37-68
Carranza FA, MG Newman , HH Takei, Klokkevold PR. 2015. Carranza’s Clinical Periodontology . Missouri: Saunders Elsevier. 12th ed. Pp. 99-607
Towaha, Juniaty. 2013. Kandungan Senyawa Kimia pada Daun Teh. Warta Penelitian dan Pengembangan Tanaman Industri.19(3)
Shen, Chwan-Li., Yeh K, James., Cao, Jay., Wang, Jia-Sheng. 2009. Green Tea and Bone Metabolism. Nutr Res J. 29(7): 437-456
Marinobu A, Biao W, Tanaka S, Horiuchi M, Jun L. 2009. Epigallocatechin-3-gallate suppresses osteoclast differentiation and ameliorates experimental arthritis in mice. Arthritis Rheum J. 58(7):2012-8
Lee JH, Jin H, Shim HE, Lee ZH. 2010. Epigallocatechin-3-gallate inhibits Osteoclastogenesis by Down-Regulating e-Fos Expression and Suppressing the Nuclear Factor-kB Signal. Molecular Pharmacology J. 77(1):17-25
Johansson,Anderson.2011. Aggregatibacter Actinomicetecomitans Leukotoxin : A Powerful Tool With Capacity to Cause Imbalance in The Host Inflammatory Respon. Journal Toxins 3:242-259.
Mariano, F.S., Sardi, J.C.O., Duque, C., Hofling, J.F., Goncalves, R.B. 2010. The Role of Immune System in teh Development of Periodontal Disease : a Brief Review. Rev Odonto Cienc. 25(3) : 300 – 5.
Prahasanti, Chiquita. 2013. Immunohistochemical Analysis of NF- KB (P50/P65) in Patient with Aggresive and Chronic Periodontitis. Indonesian Journal of Tropical and Infectious Disease.4(4):56-59
Zhao R, Kamon M, Sakamoto K. 2013. Epigallocatechin-3-gallate Interferes RANKL/RANK Signal Pathway and Induces Apoptosis during Osteoclastogenesis in RAW 264 Cell. Food and Nutrition Sciences J. 5:107-116
Madeira MFM, Cisalpino D, Wemeck SMC, Kikuchi H. 2013. MyD88 is essential for alveolar bone loss induced by Aggregatibacter actinomycetemcomitans lipopolysaccharide in mice. J Molecular Oral Microbyology. 28(6): 415-424
Widyaningrum, Naniek. 2013. Epigallocathecin-3-Gallate (EGCG) pada Daun teh Hijau sebagai Anti Jerawat. Majalah Farmasi dan Farmakologi. 17(3): 95-98
Shabri dan Dadan Rohdiana. 2016. Optimasi dan Karakterisasi Ekstrak Polifenol Teh Hijau dari Berbagai Pelarut. Jurnal penelitian Teh dan Kina. 19 (1) : 57-66
Amalia, Rizki. 2011. Perbedaan Jumlah Actinobacillus Ac inomycetemcomitans Pada Periodontitis Agresif Berdasarkan Jenis Kelamin. Majalah Universitas Sultan Agung
Byrne SJ, Dashper SG, Darby IB, Adams GG, Hoffmann B, Reynolds EC. 2009. Progression of Chronic Periodontitis can be Predicted by The Levels of Porphyromonas gingivalis and Treponema denticolan Subgingival Plaque. Oral Microbiology and Immunology J. 24: 469-477
Christianto, David. 2010. Laporan Magang Di Pt. Rumpun Sari Kemuning Ngargoyoso, Karanganyar Jawa Tengah (Quality Control Teh Hijau). Universitas Sebelas Maret Surakarta. Skripsi.
Raja M, Ummer F, Dhivakar C.P. 2014. Aggregatibacter Actinomicetecomitans-A Tooth Killer) : Review Article. Journal of Clinical and Diagnostic Research 8(8):13-16
Reddy S. 2011. Essentials of Clinical Periodontology and Periodontitics 3rd Ed. New Delhi: Jaypee Ltd. 220, 225-228.
Saraswati, Adeliana. 2015. Efektivitas Ektrak Daun Teh Hijau (Camelia sinensis) dengan NaOCl 2,5% Terhadap bakteri Enterecoccus faecalis Sebagai Alternatif Larutan Irigasi Saluran Akar. Fakultas Kedokteran Gigi Universitas Hasanuddin Makassar. Skripsi
Susin C, Haas AN, Valle PM, Oppermann RV, Albandar JM. 2010. Prevalence and Risk Indicators for Chronic Periodontitis in Adolescents and Young Adults in South Brazil. J Clin Periodontal. 38: 326-333
Tedjo Yuwono, Resty Annisa Damayanti. 2013. Dimetilsulfoksid Sebagai Enchancer Transpor Transdermal Teofilin Sediaan Gel. Jurnal Ilmiah Kefarmasian, 3(1) : 61-69
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